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Human RNase A is secreted by pancreatic acinar cells and can be neutralized by the proteolytic enzyme, secretory leukocyte protease inhibitor. Here we show that RNase A does not undergo an alternative splicing in humans as previously reported in mice. To analyse whether the unique (H3-G6-G7-F8-R9-R10-G11) domain of RNase A influences its susceptibility to proteases, the wild-type RNase A and a RNase A mutant (in which the H3-G6-G7-F8-R9-R10-G11 domain is deleted; Leu41-Glu53 replaced by Gly) were studied. Following transient expression in COS cells and in hepatoma cells, the RNase A mutant was rapidly degraded. In contrast, the wild-type protein was fully protected against proteolysis in the plasma membrane. The results suggest that the H3-G6-G7-F8-R9-R10-G11 domain of RNase A is required for its protection against proteolysis and might, therefore, be involved in the intracellular sorting of RNase A, as proposed for RNase 2 (Chang, S. C., Rabinowitz, H. J., Rebers, O. H., Fleming, D. R. and Chmielecki, M. A. (1987) Proc. Natl. Acad. Sci. U. S. A. 84, 3942-3946).Jae C. Hong/Associated Press

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Stay hungry. Stay foolish. The world is waiting for you. If you found this page useful, you may want to subscribe to the RSS Feed for updates. or. cancel. 5,. The name “Genesis” doesn’t have any special meaning, other than it’s a title of a song. The debut single of Uri Upadhyay. A stand-up comedian, and host of the popular stand-up comedy show Gareebaal. This is the official website of MegaFilmsTorrents. The best Video download manager & Video downloader. Hd videos download. Sync videos to your mobile devices. Raja Sen won the 66th National Film Award for Kannada film Ekalavya (also spelled as: Ekalavija) in two categories – best movie and best song – for the song “Nene Gedamba” sung by Dasari Narayana Rao and Bhavatharini. Also the movie was nominated for best actor and best director for Raja Sen. He is best known for his performances in films such as Jeevana Chaitra, Seetharamaiah Gari Manchure and Ammini Amma. He was awarded the 56th Filmfare Best Actor Award in 1991 for his performance in Seetharamaiah Gari Manchure. He won the 59th Filmfare Best

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