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Arsenic trioxide (As(2)O(3)) is an antineoplastic agent with therapeutic activity in the treatment of acute promyelocytic leukemia. The aim of this study was to characterize the effect of As(2)O(3) on the proliferation of human colon cancer cells. Human colon cancer cells HT-29 were treated with As(2)O(3). The cell viability was determined by the MTT assay. The apoptosis was assessed by flow cytometry, Western blotting, and reverse transcription-polymerase chain reaction. The expression of p53 was analyzed by immunohistochemistry. As(2)O(3) inhibited HT-29 cell growth in a dose-dependent manner. As(2)O(3) treatment of HT-29 cells also caused an accumulation of cells in the G1 phase of the cell cycle. The expression of p53 was
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