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([Figure 3](#F3){ref-type=”fig”}). The bi-exponential model also showed significantly better fits than the mono-exponential model (p = 0.001) and a decreased τ~1~ (p = 0.01).
Discussion
==========
The present study demonstrates the advantage of using a bi-exponential model in NIRS-based RTP of the superficial musculo-aponeurotic system (SMAS) when measured using time domain analysis and HbO~2~ concentrations. The application of the bi-exponential model improved the agreement between observed and expected HbO~2~ values, and resulted in a significant reduction of bi-exponential model R^2^, indicating that the bi-exponential model is more robust in detecting clinically relevant differences in HbO~2~ concentrations for changes in perfusion in the SMAS ([@B2]).
Previous studies using near-infrared spectroscopy (NIRS) to investigate SMAS perfusion have used single-exponential models to analyze serial changes in HbO~2~ concentrations ([@B11],[@B13]-[@B15],[@B18],[@B19],[@B23]-[@B25]). We used the bi-exponential model which was suggested to be more suitable to detect slow and rapid changes in tissue oxygenation compared with the single-exponential model in a recent NIRS perfusion study ([@B2]). However, the previous reports of HbO~2~ changes in SMAS using NIRS have mainly focused on evaluating the recovery rate of nerve function or donor site scarring ([@B13]-[@B15],[@B23],[@B24]). None of the previous studies used time domain analysis to determine whether the NIRS signal was mono-exponential or bi-exponential in nature, let alone applied a bi-exponential model in NIRS-based RTP of the SMAS.
In the literature, there are only a few studies that have applied a bi-exponential
https://colab.research.google.com/drive/1FWNWXnQYRZoALwoea2f1yBgISG0anUhO
https://colab.research.google.com/drive/1Dski8qvQr7xjreHrGnlfZIJr4Cm1n0zV
https://colab.research.google.com/drive/1-f4n1zSBm123lx4hqx0rGhGJBTYArLD0
https://colab.research.google.com/drive/1xR8eZ7esNiypzVLo1v1t9V0RSkYoXNB8
https://colab.research.google.com/drive/1OpIPnbdo2ipbUoXPnkXv_pVj4op10zE8
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